High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.

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Totani K, Matsuo I, Ihara Y, Ito Y

High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.

Bioorg Med Chem. 2006 Aug 1;14(15):5220-9. Epub 2006 May 2.

PubMed ID

16647263 [ View in PubMed

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Abstract

Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan-methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan-MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Methotrexate	Dihydrofolate reductase	Protein	Humans	Yes	Inhibitor	Details