Structural determinants of nuclear receptor assembly on DNA direct repeats.
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Rastinejad F, Perlmann T, Evans RM, Sigler PB
Structural determinants of nuclear receptor assembly on DNA direct repeats.
Nature. 1995 May 18;375(6528):203-11.
- PubMed ID
- 7746322 [ View in PubMed]
- Abstract
Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5'-AGGTCA-3' separated by one to five base pairs. The 1.9 A crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.