Molecular cloning of complementary DNA for human medullasin: an inflammatory serine protease in bone marrow cells.
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Okano K, Aoki Y, Sakurai T, Kajitani M, Kanai S, Shimazu T, Shimizu H, Naruto M
Molecular cloning of complementary DNA for human medullasin: an inflammatory serine protease in bone marrow cells.
J Biochem. 1987 Jul;102(1):13-6.
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- 2822677 [ View in PubMed]
- Abstract
Medullasin, an inflammatory serine protease in bone marrow cells, modifies the functions of natural killer cells, monocytes, and granulocytes. We have cloned a medullasin cDNA from a human acute promyelocytic cell (ML3) cDNA library using oligonucleotide probes synthesized from the information of N-terminal amino acid sequence of natural medullasin. The cDNA contained a long open reading frame encoding 237 amino acid residues beginning from the second amino acid of natural meduallasin. The deduced amino acid sequence of medullasin shows a typical serine protease structure, with 41% homology with pig elastase 1.