L-dopa and dopamine enhance the formation of aggregates under proteasome inhibition in PC12 cells.

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Citation

Yoshimoto Y, Nakaso K, Nakashima K

L-dopa and dopamine enhance the formation of aggregates under proteasome inhibition in PC12 cells.

FEBS Lett. 2005 Feb 14;579(5):1197-202. Epub 2005 Jan 21.

PubMed ID
15710413 [ View in PubMed
]
Abstract

The formation of inclusion bodies in dopaminergic neurons is associated with the pathogenesis of Parkinson's disease. In order to clarify the role of dopamine/L-dopa in the formation of protein aggregates, we investigated dopamine/L-dopa-related aggregation using an experimental inclusion model. The inhibition of tyrosine hydroxylase (TH) by alpha-methyltyrosine dramatically decreased MG132-induced aggregate formation. In addition, the inhibition of TH caused the upregulation of proteasomes in cultured cells and the dopamine/L-dopa induced non-enzymatic polymerization of ubiquitin. This inhibition did not affect cell viability. These results suggest that dopamine/L-dopa might enhance aggregate formation, and that intracellular aggregates may not be toxic to cells.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
MetyrosineTyrosine 3-monooxygenaseProteinHumans
Yes
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