Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development.

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Bourd-Boittin K, Fridman R, Fanchon S, Septier D, Goldberg M, Menashi S

Matrix metalloproteinase inhibition impairs the processing, formation and mineralization of dental tissues during mouse molar development.

Exp Cell Res. 2005 Apr 1;304(2):493-505. Epub 2005 Jan 11.

PubMed ID

15748894 [ View in PubMed

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Abstract

Organotypic cultures of embryonic mouse tooth germs were used to investigate the developmental expression and roles of MMPs in the formation and mineralization of dentin and enamel matrices. The spatially and temporally regulated expression of MMP-2, MMP-9 and MMP-20 in developing mouse tooth germs in vivo was maintained in culture. The inhibition of metalloproteinases activity by marimastat altered the morphogenesis and mineralization of the tooth germs associated with an inhibition of the activation of both MMP-20 and MMP-2. MMP inhibition deregulated the molecular processing of two major dental matrix proteins, amelogenin and dentin sialoprotein (DSP). This coincided with their accumulation and the loss of their normal distribution within the extracellular matrix, resulting in a defective mineralization of dentin and enamel matrices. These findings demonstrate the critical role of MMPs in the processing and maturation of the dental matrix.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Marimastat	72 kDa type IV collagenase	Protein	Humans	Yes	Inhibitor	Details