Interactions of pure beta gamma-subunits of G-proteins with purified beta 1-adrenoceptor.

Article Details

Citation

Im MJ, Holzhofer A, Bottinger H, Pfeuffer T, Helmreich EJ

Interactions of pure beta gamma-subunits of G-proteins with purified beta 1-adrenoceptor.

FEBS Lett. 1988 Jan 25;227(2):225-9.

PubMed ID
2828119 [ View in PubMed
]
Abstract

The role of the beta gamma-subunits in the interaction of G-proteins was examined with beta 1-adrenoceptors purified from turkey erythrocytes and pure beta gamma-subunits prepared from turkey erythrocytes and bovine brain. On a non-denaturing polyacrylamide gel, the mobility of beta gamma-subunits was increased when incubated with beta 1-adrenoceptor and the beta 1-adrenergic agonist 1-(-)-isoproterenol, whereas on incubation with the antagonist 1-alprenolol the mobility was unchanged. Furthermore, the beta 1-adrenoceptor was retarded on a Sephadex G-50 column equilibrated with beta gamma-subunits and agonist. No retardation occurred in the presence of antagonist. These data suggest a direct interaction of activated beta 1-adrenoceptors with isolated beta gamma-subunits of G-proteins.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
AlprenololBeta-1 adrenergic receptorProteinHumans
Yes
Antagonist
Details