Interactions of pure beta gamma-subunits of G-proteins with purified beta 1-adrenoceptor.
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Im MJ, Holzhofer A, Bottinger H, Pfeuffer T, Helmreich EJ
Interactions of pure beta gamma-subunits of G-proteins with purified beta 1-adrenoceptor.
FEBS Lett. 1988 Jan 25;227(2):225-9.
- PubMed ID
- 2828119 [ View in PubMed]
- Abstract
The role of the beta gamma-subunits in the interaction of G-proteins was examined with beta 1-adrenoceptors purified from turkey erythrocytes and pure beta gamma-subunits prepared from turkey erythrocytes and bovine brain. On a non-denaturing polyacrylamide gel, the mobility of beta gamma-subunits was increased when incubated with beta 1-adrenoceptor and the beta 1-adrenergic agonist 1-(-)-isoproterenol, whereas on incubation with the antagonist 1-alprenolol the mobility was unchanged. Furthermore, the beta 1-adrenoceptor was retarded on a Sephadex G-50 column equilibrated with beta gamma-subunits and agonist. No retardation occurred in the presence of antagonist. These data suggest a direct interaction of activated beta 1-adrenoceptors with isolated beta gamma-subunits of G-proteins.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Alprenolol Beta-1 adrenergic receptor Protein Humans YesAntagonistDetails