Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen.

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Citation

Israeli RS, Powell CT, Fair WR, Heston WD

Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen.

Cancer Res. 1993 Jan 15;53(2):227-30.

PubMed ID
8417812 [ View in PubMed
]
Abstract

Recently, a novel M(r) 100,000 prostate-specific membrane glycoprotein (PSM) has been detected by the prostate-specific monoclonal antibody 7E11-C5, raised against the human prostatic carcinoma cell line LNCaP. The PSM antigen is expressed exclusively by normal and neoplastic prostate cells and metastases. We now report the molecular cloning of a full-length 2.65-kilobase complementary DNA encoding the PSM antigen from a human LNCaP complementary DNA library by polymerase chain reaction using degenerate oligonucleotide primers. Analysis of the complementary DNA sequence has revealed that a portion of the coding region, from nucleotide 1250 to 1700, has 54% homology to the human transferrin receptor mRNA. The deduced polypeptide has a putative transmembrane domain enabling the delineation of intra- and extracellular portions of this antigen. In contrast to prostate-specific antigen and prostatic acid phosphatase which are secreted proteins, PSM as an integral membrane protein may prove to be effective as a target for imaging and cytotoxic targeting modalities.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Glutamate carboxypeptidase 2Q04609Details