Two distinct cDNAs for human IMP dehydrogenase.

Article Details

Citation

Natsumeda Y, Ohno S, Kawasaki H, Konno Y, Weber G, Suzuki K

Two distinct cDNAs for human IMP dehydrogenase.

J Biol Chem. 1990 Mar 25;265(9):5292-5.

PubMed ID
1969416 [ View in PubMed
]
Abstract

IMP dehydrogenase (EC 1.1.1.205), the rate-limiting enzyme of de novo GTP biosynthesis, is a promising target in antileukemic chemotherapy. We have isolated two distinct cDNA clones (types I and II) encoding IMP dehydrogenase from a human spleen cDNA library. Both clones encode closely related proteins of 514 residues showing 84% sequence identity. Northern hybridization analyses of poly(A)+ RNA from human normal leukocytes and human ovarian tumors demonstrated a striking contrast in mRNA expression in that type I mRNA is the main species in normal leukocytes and type II predominates over type I in the tumor. This is the first report suggesting the existence of two distinct types of human IMP dehydrogenase molecular species which may have different sensitivities to the drugs targeted against IMP dehydrogenase.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Inosine-5'-monophosphate dehydrogenase 2P12268Details
Inosine-5'-monophosphate dehydrogenase 1P20839Details