Primary structure of the alpha-subunit of human Na,K-ATPase deduced from cDNA sequence.
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Kawakami K, Ohta T, Nojima H, Nagano K
Primary structure of the alpha-subunit of human Na,K-ATPase deduced from cDNA sequence.
J Biochem. 1986 Aug;100(2):389-97.
- PubMed ID
- 2430951 [ View in PubMed]
- Abstract
Clones carrying cDNA sequences for the alpha-subunit of the Na,K-ATPase from HeLa cells have been isolated. Nucleotide sequence analysis of the cloned cDNA has revealed the primary structure of this polypeptide, which consists of 1,023 amino acids. The alpha-subunit of the human Na,K-ATPase exhibited 87% homology with its Torpedo counterpart and 98% homology with its sheep counterpart. The six putative transmembrane segments M1-M6 showed higher conservation than the total segments. Total genomic Southern hybridization indicated the existence of at most two copies, possibly only one, of the gene encoding the Na,K-ATPase alpha-subunit in the human genome.