Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
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Shull MM, Lingrel JB
Multiple genes encode the human Na+,K+-ATPase catalytic subunit.
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4039-43.
- PubMed ID
- 3035563 [ View in PubMed]
- Abstract
A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.