Indirect immunofluorescence studies on the steroid-producing activity of hamster ova.

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Suzuki S, Endo Y, Tanaka S, Iizuka R

Indirect immunofluorescence studies on the steroid-producing activity of hamster ova.

Am J Obstet Gynecol. 1984 Jan 1;148(1):76-85.

PubMed ID

6362415 [ View in PubMed

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Abstract

Steroidogenesis in the unfertilized hamster ova was investigated by indirect immunofluorescence study. delta 5-3 beta-Hydroxysteroid dehydrogenase (delta 5-3 beta-HSD) activity was found in hamster follicular and ovulatory ova with pregnenolone used as the substrate. A significant quantity of 17 beta-estradiol was detected in the ooplasm of follicular and ovulatory ova. No progesterone was found. The activity of delta 5-3 beta-HSD in follicular and ovulatory ova was inhibited by preincubation with 10 IU of human chorionic gonadotropin (hCG) in 1 ml of phosphate buffer solution. Trilostane, a potent new inhibitor of the delta 5-3 beta-HSD system, was found to inhibit the activity of delta 5-3 beta-HSD in ovulatory ova in 1 ml of phosphate buffer solution at a concentration of 10(-7)M. After these results were obtained, steroidogenesis of hamster ova was suggested in an indirect immunofluorescence study, and the implications of steroidogenesis for oocyte maturation and subsequent fertilization are discussed.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Trilostane	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1	Protein	Humans	Yes	Inhibitor	Details
Trilostane	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2	Protein	Humans	Yes	Inhibitor	Details