Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.
Article Details
- CitationCopy to clipboard
Wei J, Goldberg MB, Burland V, Venkatesan MM, Deng W, Fournier G, Mayhew GF, Plunkett G 3rd, Rose DJ, Darling A, Mau B, Perna NT, Payne SM, Runyen-Janecky LJ, Zhou S, Schwartz DC, Blattner FR
Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.
Infect Immun. 2003 May;71(5):2775-86.
- PubMed ID
- 12704152 [ View in PubMed]
- Abstract
We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with much less horizontally transferred DNA and lacking 357 genes present in E. coli. The strain is distinctive in its large complement of insertion sequences, with several genomic rearrangements mediated by insertion sequences, 12 cryptic prophages, 372 pseudogenes, and 195 S. flexneri-specific genes. The 2457T genome was also compared with that of a recently sequenced S. flexneri 2a strain, 301. Our data are consistent with Shigella being phylogenetically indistinguishable from E. coli. The S. flexneri-specific regions contain many genes that could encode proteins with roles in virulence. Analysis of these will reveal the genetic basis for aspects of this pathogenic organism's distinctive lifestyle that have yet to be explained.
DrugBank Data that Cites this Article
- Polypeptides
Name UniProt ID 50S ribosomal protein L10 P0A7J6 Details UDP-N-acetylglucosamine 1-carboxyvinyltransferase P0A751 Details 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase P62619 Details ADP-ribose pyrophosphatase P83844 Details Transaldolase B P0A872 Details Fatty acid metabolism regulator protein P0A8V9 Details 2-dehydro-3-deoxyphosphooctonate aldolase P0A716 Details Cell division protein ZipA Q83QN9 Details Outer-membrane lipoprotein LolB P61322 Details S-adenosylmethionine synthase P0A820 Details Aspartate 1-decarboxylase P0A793 Details 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase Q83LD8 Details Threonine--tRNA ligase P0A8M5 Details Peptide methionine sulfoxide reductase MsrA P0A745 Details Ribose-5-phosphate isomerase A P0A7Z3 Details N-acetylneuraminate lyase P0A6L6 Details Aquaporin Z O68874 Details Ribosome-recycling factor P0A808 Details Phosphopantetheine adenylyltransferase P0A6I8 Details Thiosulfate sulfurtransferase GlpE P0A6V7 Details L-fucose isomerase P69923 Details GTP cyclohydrolase 1 P0A6T8 Details Adenylosuccinate synthetase Q83P33 Details 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase P0A6Q5 Details Serine hydroxymethyltransferase P0A827 Details Elongation factor Tu Q83JC4 Details Formyl-CoA:oxalate CoA-transferase P69903 Details Methylglyoxal synthase P0A733 Details Glucose-1-phosphate thymidylyltransferase 2 P61888 Details Aspartate carbamoyltransferase catalytic chain P0A789 Details Thymidylate kinase P0A721 Details tRNA (guanine-N(1)-)-methyltransferase P0A876 Details Nicotinate-nucleotide adenylyltransferase P0A753 Details Trigger factor P0A852 Details Protein RecA P0A7G9 Details ATP-dependent protease ATPase subunit HslU P0A6H7 Details Geranyltranstransferase (Farnesyldiphosphate synthase) A0A0H2UXE9 Details