Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.

Article Details

Citation

Wei J, Goldberg MB, Burland V, Venkatesan MM, Deng W, Fournier G, Mayhew GF, Plunkett G 3rd, Rose DJ, Darling A, Mau B, Perna NT, Payne SM, Runyen-Janecky LJ, Zhou S, Schwartz DC, Blattner FR

Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.

Infect Immun. 2003 May;71(5):2775-86.

PubMed ID
12704152 [ View in PubMed
]
Abstract

We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with much less horizontally transferred DNA and lacking 357 genes present in E. coli. The strain is distinctive in its large complement of insertion sequences, with several genomic rearrangements mediated by insertion sequences, 12 cryptic prophages, 372 pseudogenes, and 195 S. flexneri-specific genes. The 2457T genome was also compared with that of a recently sequenced S. flexneri 2a strain, 301. Our data are consistent with Shigella being phylogenetically indistinguishable from E. coli. The S. flexneri-specific regions contain many genes that could encode proteins with roles in virulence. Analysis of these will reveal the genetic basis for aspects of this pathogenic organism's distinctive lifestyle that have yet to be explained.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
50S ribosomal protein L10P0A7J6Details
UDP-N-acetylglucosamine 1-carboxyvinyltransferaseP0A751Details
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseP62619Details
ADP-ribose pyrophosphataseP83844Details
Transaldolase BP0A872Details
Fatty acid metabolism regulator proteinP0A8V9Details
2-dehydro-3-deoxyphosphooctonate aldolaseP0A716Details
Cell division protein ZipAQ83QN9Details
Outer-membrane lipoprotein LolBP61322Details
S-adenosylmethionine synthaseP0A820Details
Aspartate 1-decarboxylaseP0A793Details
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseQ83LD8Details
Threonine--tRNA ligaseP0A8M5Details
Peptide methionine sulfoxide reductase MsrAP0A745Details
Ribose-5-phosphate isomerase AP0A7Z3Details
N-acetylneuraminate lyaseP0A6L6Details
Aquaporin ZO68874Details
Ribosome-recycling factorP0A808Details
Phosphopantetheine adenylyltransferaseP0A6I8Details
Thiosulfate sulfurtransferase GlpEP0A6V7Details
L-fucose isomeraseP69923Details
GTP cyclohydrolase 1P0A6T8Details
Adenylosuccinate synthetaseQ83P33Details
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseP0A6Q5Details
Serine hydroxymethyltransferaseP0A827Details
Elongation factor TuQ83JC4Details
Formyl-CoA:oxalate CoA-transferaseP69903Details
Methylglyoxal synthaseP0A733Details
Glucose-1-phosphate thymidylyltransferase 2P61888Details
Aspartate carbamoyltransferase catalytic chainP0A789Details
Thymidylate kinaseP0A721Details
tRNA (guanine-N(1)-)-methyltransferaseP0A876Details
Nicotinate-nucleotide adenylyltransferaseP0A753Details
Trigger factorP0A852Details
Protein RecAP0A7G9Details
ATP-dependent protease ATPase subunit HslUP0A6H7Details
Geranyltranstransferase (Farnesyldiphosphate synthase)A0A0H2UXE9Details