Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins.

Article Details

Citation

Favaloro V, Spasic M, Schwappach B, Dobberstein B

Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins.

J Cell Sci. 2008 Jun 1;121(11):1832-40. doi: 10.1242/jcs.020321. Epub 2008 May 13.

PubMed ID
18477612 [ View in PubMed
]
Abstract

Tail-anchored (TA) proteins are characterised by a C-terminal transmembrane region that mediates post-translational insertion into the membrane of the endoplasmic reticulum (ER). We have investigated the requirements for membrane insertion of three TA proteins, RAMP4, Sec61beta and cytocrome b5. We show here that newly synthesised RAMP4 and Sec61beta can accumulate in a cytosolic, soluble complex with the ATPase Asna1 before insertion into ER-derived membranes. Membrane insertion of these TA proteins is stimulated by ATP, sensitive to redox conditions and blocked by alkylation of SH groups by N-ethylmaleimide (NEM). By contrast, membrane insertion of cytochrome b5 is not found to be mediated by Asna1, not stimulated by ATP and not affected by NEM or an oxidative environment. The Asna1-mediated pathway of membrane insertion of RAMP4 and Sec61beta may relate to functions of these proteins in the ER stress response.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
ATPase ASNA1O43681Details