Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments.

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Amanuma K, Takeda H, Amanuma H, Aoki Y

Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments.

Nat Biotechnol. 2000 Jan;18(1):62-5.

PubMed ID

10625393 [ View in PubMed

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Abstract

We have established a transgenic zebrafish line carrying a shuttle vector plasmid (pML4) for detecting mutagens in aquatic environments. The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA. To evaluate the system, we treated embryos of the transgenic fish with N-ethyl-N-nitrosourea (ENU), which induces a dose-dependent increase in the mutation frequency of the target gene. The mutation spectrum was consistent with the proposed mechanism of ENU mutagenesis. Similarly, treating the embryos with benzo[a]pyrene or 2-amino-3, 8-dimethylimidazo[4,5- f]quinoxaline, which are found in naturally polluted water, significantly increased the frequency of mutations in the target gene.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Kanamycin	30S ribosomal protein S12	Protein	Escherichia coli (strain K12)	Yes	Inhibitor	Details