Isolation of an evolutionarily conserved epidermal growth factor receptor cDNA from human A431 carcinoma cells.

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Citation

Simmen FA, Gope ML, Schulz TZ, Wright DA, Carpenter G, O'Malley BW

Isolation of an evolutionarily conserved epidermal growth factor receptor cDNA from human A431 carcinoma cells.

Biochem Biophys Res Commun. 1984 Oct 15;124(1):125-32.

PubMed ID
6093780 [ View in PubMed
]
Abstract

Complementary DNA corresponding to total poly(A)+-RNA from the human A431 epidermoid carcinoma cell line was cloned in the phage expression vector lambda gt 11. An epidermal growth factor (EGF) receptor cDNA clone was obtained by screening of the expression library with a rabbit polyclonal antibody (IgG), raised to the purified A431 EGF receptor, in combination with [125I]protein A of S. aureus. The cloned cDNA was able to select, by hybridization, messenger RNA which was translated in Xenopus oocytes and yielded an immunoprecipitable EGF receptor protein of Mr = 160,000. The insert of this cDNA (phEGFR-1), is approximately 880 base pairs in length and encodes the carboxyterminal portion of the EGF receptor protein. Its sequence is evolutionarily conserved among vertebrates as shown by hybridization to unique chromosomal DNA sequences from human, baboon, dog, rat, mouse and frog.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Epidermal growth factor receptorP00533Details