Tyrosine phosphorylation controls PCNA function through protein stability.

Article Details

Citation

Wang SC, Nakajima Y, Yu YL, Xia W, Chen CT, Yang CC, McIntush EW, Li LY, Hawke DH, Kobayashi R, Hung MC

Tyrosine phosphorylation controls PCNA function through protein stability.

Nat Cell Biol. 2006 Dec;8(12):1359-68. Epub 2006 Nov 19.

PubMed ID
17115032 [ View in PubMed
]
Abstract

The proliferating cell nuclear antigen (PCNA) is an essential protein for DNA replication and damage repair. How its function is controlled remains an important question. Here, we show that the chromatin-bound PCNA protein is phosphorylated on Tyr 211, which is required for maintaining its function on chromatin and is dependent on the tyrosine kinase activity of EGF receptor (EGFR) in the nucleus. Phosphorylation on Tyr 211 by EGFR stabilizes chromatin-bound PCNA protein and associated functions. Consistently, increased PCNA Tyr 211 phosphorylation coincides with pronounced cell proliferation, and is better correlated with poor survival of breast cancer patients, as well as nuclear EGFR in tumours, than is the total PCNA level. These results identify a novel nuclear mechanism linking tyrosine kinase receptor function with the regulation of the PCNA sliding clamp.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Epidermal growth factor receptorP00533Details
Proliferating cell nuclear antigenP12004Details