Cloning and characterization of the human V3 pituitary vasopressin receptor.

Article Details

Citation

de Keyzer Y, Auzan C, Lenne F, Beldjord C, Thibonnier M, Bertagna X, Clauser E

Cloning and characterization of the human V3 pituitary vasopressin receptor.

FEBS Lett. 1994 Dec 19;356(2-3):215-20.

PubMed ID
7805841 [ View in PubMed
]
Abstract

Arginine-vasopressin (AVP) plays a determinant role in the normal ACTH response to stress in mammals. We cloned a human cDNA coding a 424 amino acid G-protein coupled receptor structurally related to the vasopressin/oxytocin receptor family. When expressed in COS cells, this receptor binds AVP with a high affinity (Kd = 0.55 +/- 0.13 nM) and is functionally coupled to phospholipase C. Competition studies with peptidic or non peptidic AVP analogues reveal that it is pharmacologically distinct from V1a and V2 AVP receptors and therefore it is designated V3. RT-PCR analysis shows that the human V3 receptor is expressed in normal pituitary and also in kidney, but is undetectable in liver, myometrium and adrenal gland. Northern blot analysis reveals a approximately 4.8 kb messenger in human corticotropic pituitary adenomas.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Vasopressin V1b receptorP47901Details