Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation.
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Hosaka K, Tanaka S, Nikawa J, Yamashita S
Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation.
FEBS Lett. 1992 Jun 15;304(2-3):229-32.
- PubMed ID
- 1618328 [ View in PubMed]
- Abstract
A human choline kinase cDNA was cloned by complementation of the yeast choline kinase mutation, cki, from a human glioblastoma cDNA expression library. The deduced sequence of the human enzyme comprised 456 amino acids with a calculated relative molecular mass of 52,065. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not much in the amino-terminal region.