New topoisomerase essential for chromosome segregation in E. coli.

Article Details

Citation

Kato J, Nishimura Y, Imamura R, Niki H, Hiraga S, Suzuki H

New topoisomerase essential for chromosome segregation in E. coli.

Cell. 1990 Oct 19;63(2):393-404.

PubMed ID
2170028 [ View in PubMed
]
Abstract

The nucleotide sequence of the parC gene essential for chromosome partition in E. coli was determined. The deduced amino acid sequence was homologous to that of the A subunit of gyrase. We found another new gene coding for about 70 kd protein. The gene was sequenced, and the deduced amino acid sequence revealed that the gene product was homologous to the gyrase B subunit. Mutants of this gene were isolated and showed the typical Par phenotype at nonpermissive temperature; thus the gene was named parE. Enhanced relaxation activity of supercoiled plasmid molecules was detected in the combined crude cell lysates prepared from the ParC and ParE overproducers. A topA mutation defective in topoisomerase I could be compensated by increasing both the parC and the parE gene dosage. It is suggested that the parC and parE genes code for the subunits of a new topoisomerase, named topo IV.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
DNA topoisomerase 4 subunit AP0AFI2Details
DNA topoisomerase 4 subunit BP20083Details