Human T lymphocytes express a protein-tyrosine kinase homologous to p56LSTRA.
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Trevillyan JM, Lin Y, Chen SJ, Phillips CA, Canna C, Linna TJ
Human T lymphocytes express a protein-tyrosine kinase homologous to p56LSTRA.
Biochim Biophys Acta. 1986 Oct 10;888(3):286-95.
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- 3489486 [ View in PubMed]
- Abstract
We have used oligonucleotide probes, based on a portion of the p60v-src autophosphorylation sequence, Glu-Asp-Asn-Glu-Tyr-Thr, to identify and characterize a cDNA from the human T-leukemia cell line, JURKAT. The JURKAT cDNA (designated ptk-JURKAT) was homologous to but distinct from the src, yes and fgr oncogenes, which encode protein-tyrosine kinases (ATP:protein phosphotransferase, EC 2.7.1.37). The ptk-JURKAT cDNA hybridized with a 2.2 kb RNA transcript from JURKAT cells and the human T-cell lymphoma line, MOLT-4, but failed to identify any transcript in two human B-cell lymphoma lines or a human erythroid-myeloid leukemia line, K562. Recently the nucleotide sequence has been established for the murine lymphocyte protein tyrosine kinase, p56LSTRA. The ptk-JURKAT cDNA appears to encode the human homolog of p56LSTRA.