Molecular cloning of a novel melanocortin receptor.

Article Details

Citation

Gantz I, Konda Y, Tashiro T, Shimoto Y, Miwa H, Munzert G, Watson SJ, DelValle J, Yamada T

Molecular cloning of a novel melanocortin receptor.

J Biol Chem. 1993 Apr 15;268(11):8246-50.

PubMed ID
8463333 [ View in PubMed
]
Abstract

Using the technique of the polymerase chain reaction primed with oligonucleotides based on the homologous transmembrane regions of seven transmembrane G protein-linked receptors, we isolated three full-length human genes that encode a novel subgroup of this receptor family. Recently, two of these receptors were identified as specific for alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropic hormone. We report the molecular cloning and pharmacologic characterization of a third member of this subgroup. The gene for this receptor encodes a protein of 361 amino acids in length. Its pharmacology characterizes it as an MSH receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. By Northern blot hybridization and polymerase chain reaction, it is expressed in brain, placental, and gut tissues but not in melanoma cells or in the adrenal gland. These findings may yield insight into the physiology of peptides derived from pro-opiomelanocortin post-translational processing.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Adrenocorticotropic hormone receptorQ01718Details
Melanocyte-stimulating hormone receptorQ01726Details
Melanocortin receptor 3P41968Details