Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32.

Article Details

Citation
Copy to clipboard

Groot Kormelink PJ, Luyten WH

Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32.

FEBS Lett. 1997 Jan 6;400(3):309-14.

PubMed ID
9009220 [ View in PubMed
]
Abstract

Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except for the beta2, all the other cloned cDNAs showed differences with the published sequences. Northern blots show expression of the nAChR subunits alpha3,7 and beta2,4 in the human neuroblastoma cell line SH-SY5Y, with intensity of the hybridisation signal decreasing in the order alpha3 > beta4 > beta2 > alpha7.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Neuronal acetylcholine receptor subunit alpha-4P43681Details
Neuronal acetylcholine receptor subunit beta-2P17787Details
Neuronal acetylcholine receptor subunit alpha-7P36544Details
Neuronal acetylcholine receptor subunit beta-4P30926Details
Neuronal acetylcholine receptor subunit alpha-3P32297Details
Neuronal acetylcholine receptor subunit alpha-5P30532Details
Neuronal acetylcholine receptor subunit beta-3Q05901Details