[Screening of short peptides binding to cell surface interleukin-2 receptor alpha chain].

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Liu BY, Zhu P, Luo HB, Fu N

[Screening of short peptides binding to cell surface interleukin-2 receptor alpha chain].

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jul;26(7):971-4.

PubMed ID

16864089 [ View in PubMed

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Abstract

OBJECTIVE: To screen and characterize the short peptides which bind specifically to interleukin-2 (IL-2) receptor alpha chain (IL-2Ralpha) for acquisition of small antagonists for blocking the binding of IL-2 with IL-2Ralpha. METHODS: 12-mer phage displayed peptide library was screened with the target cells of MT-2 cells which expressed IL-2Ralpha at high levels. The binding phage clones were eluted by anti-IL-2Ralpha monoclonal antibody. After 3 rounds of screening, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences of the positive clones were deduced from the DNA sequences. RESULTS: Seven positive clones were screened out of the 17 phage clones bound to MT-2 cells. The positive clone M15 could bind specifically to MT-2 cell and PHA-activated peripheral blood monouclear cells. Amino acid sequence analysis identified 6 sequences, all of which contained hydrophilic residues, and 5 of these 6 sequences included Tyr, Phe and Leu conservative residues. CONCLUSION: The peptide sequences containing Tyr, Phe conservative residues identified in this study can bind to cell surface IL-2Ralpha.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Aldesleukin	Interleukin-2 receptor subunit alpha	Protein	Humans	Yes	Agonist Modulator	Details