Serendipitous cloning of a mitochondrial cDNA and its polymorphism.
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Spurr NK, Bodmer WF
Serendipitous cloning of a mitochondrial cDNA and its polymorphism.
Mol Biol Med. 1984 Aug;2(4):239-49.
- PubMed ID
- 6100559 [ View in PubMed]
- Abstract
A cDNA clone p5x23 was isolated from a library prepared from membrane bound mRNA from a human lymphoblastoid cell line. This clone was shown to detect a restriction fragment length polymorphism with the enzyme MspI, with frequency for the minor variant of about 10% in a Caucasian population. The clone apparently only detected homozygotes, and did not hybridize to any of a panel of human-mouse somatic cell hybrids containing all human chromosomes in various combinations. Sequence analysis of the clone showed that it encoded a part of the 3' end of the human mitochondrial cytochrome b gene.