Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells.
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Westley BR, May FE
Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells.
Nucleic Acids Res. 1987 May 11;15(9):3773-86.
- PubMed ID
- 3588310 [ View in PubMed]
- Abstract
A cDNA library was constructed from the mRNA of the ZR-75 oestrogen responsive human breast cancer cell line and screened for oestrogen regulated mRNA sequences. Of the recombinants isolated, 30 contained cDNA that corresponded to a single mRNA species of 2.1 kb that was induced between 10 and 15 fold by oestradiol treatment. The sequence of the entire open reading frame and 3' non-coding region of the mRNA was determined and shown to encode the aspartyl protease cathepsin D. The induction of cathepsin D mRNA is specific for oestrogen and is maximal at 3 X 10(-10) M. Cathepsin D mRNA levels were increased by oestrogen in 3 oestrogen responsive breast cancer cell lines. Cathepsin D mRNA was expressed but not regulated in an oestrogen receptor negative breast tumour cell line, BT 20, and in 2 other malignant cell lines, Hela and A431.