Cloning and sequence of cDNA coding for alpha 1-antitrypsin.
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Kurachi K, Chandra T, Degen SJ, White TT, Marchioro TL, Woo SL, Davie EW
Cloning and sequence of cDNA coding for alpha 1-antitrypsin.
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6826-30.
- PubMed ID
- 7031661 [ View in PubMed]
- Abstract
Recombinant plasmids containing human and baboon cDNA have been screened for alpha 1-antitrypsin, a major serine protease inhibitor present in blood. One plasmid, designated pBa alpha 1a2, was found to contain a cDNA insert of 1352 base pairs coding for the baboon inhibitor. It included 45 nucleotides that code for 15 amino acids present in the amino-terminal signal sequence of the protein, 1182 nucleotides that code for 394 amino acids in the mature protein, a stop codon, and a noncoding region of 76 nucleotides. Comparison of the amino acid sequences of baboon alpha 1-antitrypsin, human antithrombin III, and chicken ovalbumin indicated that these three proteins are about 230% homologous. A second plasmid, designated pH alpha 1a1, was found to contain a human cDNA insert of 306 base pairs. This plasmid coded for 69 amino acids present in the carboxyl-terminal region of human alpha 1-antitrypsin. The human and baboon cDNAs and their amino acid sequences are greater than 96% homologous.