Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.

Article Details

Citation

Wichroski MJ, Robb GB, Rana TM

Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.

PLoS Pathog. 2006 May;2(5):e41. Epub 2006 May 12.

PubMed ID
16699599 [ View in PubMed
]
Abstract

APOBEC3G is an antiviral host factor capable of inhibiting the replication of both exogenous and endogenous retroviruses as well as hepatitis B, a DNA virus that replicates through an RNA intermediate. To gain insight into the mechanism whereby APOBEC3G restricts retroviral replication, we investigated the subcellular localization of the protein. Herein, we report that APOBEC3G localizes to mRNA processing (P) bodies, cytoplasmic compartments involved in the degradation and storage of nontranslating mRNAs. Biochemical analysis revealed that APOBEC3G localizes to a ribonucleoprotein complex with other P-body proteins which have established roles in cap-dependent translation (eIF4E and eIF4E-T), translation suppression (RCK/p54), RNA interference-mediated post-transcriptional gene silencing (AGO2), and decapping of mRNA (DCP2). Similar analysis with other APOBEC3 family members revealed a potential link between the localization of APOBEC3G and APOBEC3F to a common ribonucleoprotein complex and P-bodies with potent anti-HIV-1 activity. In addition, we present evidence suggesting that an important role for HIV-1 Vif, which subverts both APOBEC3G and APOBEC3F antiviral function by inducing their degradation, could be to selectively remove these proteins from and/or restrict their localization to P-bodies. Taken together, the results of this study reveal a novel link between innate immunity against retroviruses and P-bodies suggesting that APOBEC3G and APOBEC3F could function in the context of P-bodies to restrict HIV-1 replication.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Eukaryotic translation initiation factor 4EP06730Details
Probable ATP-dependent RNA helicase DDX6P26196Details