Carboxyl terminal analysis of human B-48 protein confirms the novel mechanism proposed for chain termination.
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Hardman DA, Protter AA, Schilling JW, Kane JP
Carboxyl terminal analysis of human B-48 protein confirms the novel mechanism proposed for chain termination.
Biochem Biophys Res Commun. 1987 Dec 31;149(3):1214-9.
- PubMed ID
- 3426612 [ View in PubMed]
- Abstract
In this study we have confirmed the presence of a single base difference between intestinal mRNA coding for B-48 and hepatic mRNA coding for B-100, which results in the substitution of a stop codon (UAA) for a glutamine codon (CAA) at a point corresponding to amino acid residue 2153 in the B-100 sequence. Based on this finding, B-48 is predicted to terminate at residue 2152 with the sequence ... Met Ile. To confirm this finding at the protein level, B-48 and B-100 were each digested with cyanogen bromide and the digestion products were analysed for the presence of isoleucine. Isoleucine was found only in cyanogen bromide digests of B-48 confirming that only B-48 terminates with the predicted amino acid sequence ... Met Ile.