Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.
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Navajas M, Laurent AM, Moreel JF, Ragab A, Cambou JP, Cuny G, Cambien F, Roizes G
Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.
Hum Genet. 1990 Nov;86(1):91-3.
- PubMed ID
- 1979313 [ View in PubMed]
- Abstract
The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual.