Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a patient with gastric cancer.

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Maeda H, Matsumura Y, Kato H

Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a patient with gastric cancer.

J Biol Chem. 1988 Nov 5;263(31):16051-4.

PubMed ID
3182782 [ View in PubMed
]
Abstract

Kinins in the ascitic fluid from a patient with gastric cancer were purified by gel filtration and reversed-phase high-performance liquid chromatography (HPLC). Two fractions (fractions I and II) showed kinin activity. Fraction I did not correspond to either bradykinin or other known kinins, whereas fraction II corresponded to bradykinin. Fraction I contained 8 amino acid residues from bradykinin minus 1 proline plus 1 additional hydroxyproline. Sequence analysis of fraction I showed that the proline at the third amino acid residue of bradykinin was replaced by hydroxyproline. The retention time of fraction I on reversed-phase HPLC was exactly the same as that of synthetic [hydroxyprolyl3]bradykinin (Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg) and was distinguishable from des-Pro3-bradykinin. Thus, these results demonstrate for the first time the presence of [hydroxyprolyl3]bradykinin in vivo. This is also the first report of the presence of bradykinin in human tumor ascites.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Kininogen-1P01042Details