Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.
Article Details
- CitationCopy to clipboard
Mott HR, Driscoll PC, Boyd J, Cooke RM, Weir MP, Campbell ID
Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.
Biochemistry. 1992 Aug 25;31(33):7741-4.
- PubMed ID
- 1510960 [ View in PubMed]
- Abstract
Recombinant 15N-labeled human interleukin 2 (IL-2) has been studied by 2D and 3D NMR using uniformly 15N-labeled protein. Assignment of the backbone resonances has enabled the secondary structure of the protein to be defined. The secondary structure was found to consist of four alpha-helical regions and a short section of antiparallel beta-sheet. This structure is more similar to recent published structures of interleukin 4 and granulocyte-macrophage colony-stimulating factor than to a structure of IL-2 previously obtained from low-resolution X-ray diffraction data.