Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte.
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Seki T, Ihara I, Sugimura A, Shimonishi M, Nishizawa T, Asami O, Hagiya M, Nakamura T, Shimizu S
Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte.
Biochem Biophys Res Commun. 1990 Oct 15;172(1):321-7.
- PubMed ID
- 2145836 [ View in PubMed]
- Abstract
Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe. Nucleotide and deduced amino acid sequences were analyzed for two of four clones obtained. One of them contained an open reading frame coding for a polypeptide chain of 728 amino acid residues like that of cDNA clone derived from human liver. In another clone a spontaneous deletion of 15 base pairs was found within the coding sequence. When expressed transiently using COS-1 cells both clones produced protein with similar biological activity against rat hepatocyte in vitro.