Hepatocyte growth factor is linked by O-glycosylated oligosaccharide on the alpha chain.
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Shimizu N, Hara H, Sogabe T, Sakai H, Ihara I, Inoue H, Nakamura T, Shimizu S
Hepatocyte growth factor is linked by O-glycosylated oligosaccharide on the alpha chain.
Biochem Biophys Res Commun. 1992 Dec 30;189(3):1329-35.
- PubMed ID
- 1482348 [ View in PubMed]
- Abstract
The glycosylation site and the structure of O-glycosylated oligosaccharide of recombinant human HGF were investigated. N-acetylgalactosamine (GalNAc) in the alpha chain suggested the presence of O-glycosylated oligosaccharide. Sugar analysis and amino acid sequence analysis of peptide fragments produced by limited degradation revealed that O-glycosylated oligosaccharide linked to Thr445 of the alpha chain. The molecular weight of the oligosaccharide was determined with ion spray mass spectrometry. From these studies, the structure of the O-glycosylated oligosaccharide on the alpha chain of HGF was concluded as [formula: see text].