Purification of a young age-related glycoprotein (Ugl-Y) from normal human urine.

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Citation

Iida R, Yasuda T, Kishi K

Purification of a young age-related glycoprotein (Ugl-Y) from normal human urine.

J Biochem. 1987 Feb;101(2):357-63.

PubMed ID
3584091 [ View in PubMed
]
Abstract

Ugl-Y is a glycoprotein that is detected in normal urine samples from young men and women aged 0 to 17 years. It was purified by ammonium sulfate precipitation and various column chromatographies including affinity chromatography using anti-adult urine antibody coupled to Sepharose 4B. The homogeneity of the glycoprotein was confirmed by polyacrylamide gel electrophoresis, isoelectric focusing, column chromatography on Sephadex G-75, and the precipitation reaction with anti-Ugl-Y antibody. It was shown to have a molecular weight of 29,000 by gel filtration, and to contain 5.2% neutral sugars (mannose and galactose) and 4% hexosamine (glucosamine). Amino acid analysis of the glycoprotein indicated high contents of acidic and hydroxylic amino acids. Its origin is unknown.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
FibronectinP02751Details