A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.

Article Details


Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.

A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.

DNA Res. 1996 Dec 31;3(6):363-77.

PubMed ID
9097039 [ View in PubMed

The 569,750 base pair sequence corresponding to the 28.0-40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in and near the replication termination region which had been thought to be genetically silent. Those included a cluster of genes for fatty acid beta-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes and a DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, and a number of genes originated probably from plasmids.

DrugBank Data that Cites this Article

NameUniProt ID
Primary amine oxidaseP46883Details
NH(3)-dependent NAD(+) synthetaseP18843Details
Pyridoxamine kinaseP77150Details
Cysteine desulfuraseP77444Details
Tryptophan biosynthesis protein TrpCFP00909Details
Gamma-aminobutyraldehyde dehydrogenaseP77674Details
Tautomerase PptAP31992Details
Orotidine 5'-phosphate decarboxylaseP08244Details
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB componentP69795Details
Fumarate hydratase class IIP05042Details
DNA topoisomerase 1P06612Details
7-alpha-hydroxysteroid dehydrogenaseP0AET8Details
Enoyl-[acyl-carrier-protein] reductase [NADH] FabIP0AEK4Details
Tyrosine--tRNA ligaseP0AGJ9Details
Glyceraldehyde-3-phosphate dehydrogenase AP0A9B2Details
Glutathione S-transferase GstAP0A9D2Details
Pyridoxine/pyridoxamine 5'-phosphate oxidaseP0AFI7Details
Probable pyruvate-flavodoxin oxidoreductaseP52647Details
FMN-dependent NADH-azoreductaseP41407Details
Riboflavin synthaseP0AFU8Details
Outer membrane protein WP0A915Details
Protein TonBP02929Details
Lactaldehyde dehydrogenaseP25553Details
Formate dehydrogenase, nitrate-inducible, major subunitP24183Details
Formate dehydrogenase, nitrate-inducible, iron-sulfur subunitP0AAJ3Details
Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunitP0AEK7Details
Medium-chain fatty-acid--CoA ligaseP38135Details