Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.

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Yura T, Mori H, Nagai H, Nagata T, Ishihama A, Fujita N, Isono K, Mizobuchi K, Nakata A

Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region.

Nucleic Acids Res. 1992 Jul 11;20(13):3305-8.

PubMed ID
1630901 [ View in PubMed
]
Abstract

A contiguous 111,402-nucleotide sequence corresponding to the 0 to 2.4 min region of the E. coli chromosome was determined as a first step to complete structural analysis of the genome. The resulting sequence was used to predict open reading frames and to search for sequence similarity against the PIR protein database. A number of novel genes were found whose predicted protein sequences showed significant homology with known proteins from various organisms, including several clusters of genes similar to those involved in fatty acid metabolism in bacteria (e.g., betT, baiF) and higher organisms, iron transport (sfuA, B, C) in Serratia marcescens, and symbiotic nitrogen fixation or electron transport (fixA, B, C, X) in Azorhizobium caulinodans. In addition, several genes and IS elements that had been mapped but not sequenced (e.g., leuA, B, C, D) were identified. We estimate that about 90 genes are represented in this region of the chromosome with little spacer.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
UDP-N-acetylmuramoylalanine--D-glutamate ligaseP14900Details
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseP17443Details
8-oxo-dGTP diphosphataseP08337Details
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseP22188Details
4-hydroxythreonine-4-phosphate dehydrogenaseP19624Details
L-carnitine CoA-transferaseP31572Details
4-hydroxy-tetrahydrodipicolinate reductaseP04036Details
Dihydrofolate reductaseP0ABQ4Details
Arabinose operon regulatory proteinP0A9E0Details
Peptidoglycan synthase FtsIP0AD68Details
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseP62623Details