Structure of a type II thymidine kinase with bound dTTP.

Article Details


Birringer MS, Claus MT, Folkers G, Kloer DP, Schulz GE, Scapozza L

Structure of a type II thymidine kinase with bound dTTP.

FEBS Lett. 2005 Feb 28;579(6):1376-82.

PubMed ID
15733844 [ View in PubMed

The structure of human cytosolic thymidine kinase in complex with its feedback inhibitor 2'-deoxythymidine-5'-triphosphate was determined. This structure is the first representative of the type II thymidine kinases found in several pathogens. The structure deviates strongly from the known structures of type I thymidine kinases such as the Herpes simplex enzyme. It contains a zinc-binding domain with four cysteines complexing a structural zinc ion. Interestingly, the backbone atoms of the type II enzyme bind thymine via hydrogen-bonds, in contrast to type I, where side chains are involved. This results in a specificity difference exploited for antiviral therapy. The presented structure will foster the development of new drugs and prodrugs for numerous therapeutic applications.

DrugBank Data that Cites this Article

NameUniProt ID
Thymidine kinase, cytosolicP04183Details