Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid.

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Ding X, Zhang W, Cheng D, He J, Yang KL

Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid.

Biosens Bioelectron. 2012 May 15;35(1):271-6. doi: 10.1016/j.bios.2012.02.060. Epub 2012 Mar 7.

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22459587 [ View in PubMed
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Abstract

By using phage display library, we identified two highly specific oligopeptide sequences RKRIRRMMPRPS and RNRHTHLRTRPR for binding neonicotinoids such as thiacloprid and imidacloprid. The former shows high affinity for thiacloprid whereas the latter shows high affinity for imidacloprid. Surprisingly, cross binding is minimal despite the similarity of the two molecules. To develop a neonicotinoid biosensor, these two oligopeptides are synthesized and immobilized on the surface of a surface plasmon resonance (SPR) chip with a bare-gold surface. This oligopeptide functionalized SPR biosensor can rapidly detect thiacloprid and imidacloprid in buffer solutions in a real-time manner. The limit of detection (LOD) for thiacloprid and imidacloprid is 1.2 muM and 0.9 muM, respectively.

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