Global topology analysis of the Escherichia coli inner membrane proteome.

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Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G

Global topology analysis of the Escherichia coli inner membrane proteome.

Science. 2005 May 27;308(5726):1321-3.

PubMed ID
15919996 [ View in PubMed
]
Abstract

The protein complement of cellular membranes is notoriously resistant to standard proteomic analysis and structural studies. As a result, membrane proteomes remain ill-defined. Here, we report a global topology analysis of the Escherichia coli inner membrane proteome. Using C-terminal tagging with the alkaline phosphatase and green fluorescent protein, we established the periplasmic or cytoplasmic locations of the C termini for 601 inner membrane proteins. By constraining a topology prediction algorithm with this data, we derived high-quality topology models for the 601 proteins, providing a firm foundation for future functional studies of this and other membrane proteomes. We also estimated the overexpression potential for 397 green fluorescent protein fusions; the results suggest that a large fraction of all inner membrane proteins can be produced in sufficient quantities for biochemical and structural work.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Signal peptidase IP00803Details
Ammonia channelP69681Details
Multidrug efflux pump subunit AcrBP31224Details
Lactose permeaseP02920Details
Glycerol uptake facilitator proteinP0AER0Details
Osmolarity sensor protein EnvZP0AEJ4Details
Sensor protein PhoQP23837Details
Potassium-transporting ATPase ATP-binding subunitP03960Details
Respiratory nitrate reductase 1 gamma chainP11350Details
Aquaporin ZP60844Details
Succinate dehydrogenase cytochrome b556 subunitP69054Details
Succinate dehydrogenase hydrophobic membrane anchor subunitP0AC44Details
Rhomboid protease GlpGP09391Details
Fumarate reductase subunit CP0A8Q0Details
Fumarate reductase subunit DP0A8Q3Details
Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunitP0AEK7Details
Disulfide bond formation protein BP0A6M2Details
Inner membrane protein YdjMP64481Details