Sequence of the lactose permease gene.
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Buchel DE, Gronenborn B, Muller-Hill B
Sequence of the lactose permease gene.
Nature. 1980 Feb 7;283(5747):541-5.
- PubMed ID
- 6444453 [ View in PubMed]
- Abstract
The nucleotide sequence of the lacY gene coding for lactose permease (M protein) in Escherichia coli has been determined. The sequence includes the intergenic regions between the lacZ (beta-galactosidase) and lacY genes as well as the region between the lacY and lacA (transacetylase) genes. Lactose permease is predicted to consist of 417 residues (71% nonpolar), resulting in a protein with a molecular weight of 46,504. The reading frame was confirmed by the sequence of a nonsense mutation changing codon 33 from UGG to UAG.