Identification of the active site of Citrobacter freundii beta-lactamase using dansyl-penicillin.
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Yamaguchi A, Adachi H, Sawai T
Identification of the active site of Citrobacter freundii beta-lactamase using dansyl-penicillin.
FEBS Lett. 1987 Jun 22;218(1):126-30.
- PubMed ID
- 3496243 [ View in PubMed]
- Abstract
The active site sequence of a beta-lactamase encoded by chromosomal gene(s) in Citrobacter freundii GN346 was determined using dansyl-penicillin as a fluorescent probe. The tryptic digest of the labelled enzyme gave a fluorescent peptide containing 22 amino acids. The sequence of this peptide was identical to the consensus sequence of class C beta-lactamases, Gly-Ser-X-Ser-Lys. The residue labelled was the serine adjacent to the glycine. The active site sequence corresponded to positions 46-67 of the entire sequence of the Citrobacter freundii beta-lactamase determined on the basis of the DNA sequence of the structural gene [(1986) Eur. J. Biochem. 156, 441-445]. The labelled serine corresponded to Ser-64.