Integration of human immunodeficiency virus type 1 DNA in vitro.
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Farnet CM, Haseltine WA
Integration of human immunodeficiency virus type 1 DNA in vitro.
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4164-8.
- PubMed ID
- 2349226 [ View in PubMed]
- Abstract
A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.