Leukotriene A4 hydrolase in human leukocytes. Purification and properties.
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Radmark O, Shimizu T, Jornvall H, Samuelsson B
Leukotriene A4 hydrolase in human leukocytes. Purification and properties.
J Biol Chem. 1984 Oct 25;259(20):12339-45.
- PubMed ID
- 6490615 [ View in PubMed]
- Abstract
Leukotriene A4 hydrolase, a soluble enzyme catalyzing hydrolysis of the allylic epoxide leukotriene A4 to the dihydroxy acid leukotriene B4, was purified to apparent homogeneity from human leukocytes. The enzymatic reaction obeyed Michaelis-Menten saturation kinetics with respect to varying concentrations of leukotriene A4. An apparent KM value ranging between 20 and 30 microM was deduced from Eadie-Hofstee plots. Physical properties including molecular weight (68,000-70,000), amino acid composition, and aminoterminal sequence were determined. It was indicated that leukotriene A4 hydrolase is a monomeric protein, distinct from previously described epoxide hydrolases in liver.