Human complement factor B: functional properties of a recombinant zymogen of the alternative activation pathway convertase.
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Schwaeble W, Luttig B, Sokolowski T, Estaller C, Weiss EH, Meyer zum Buschenfelde KH, Whaley K, Dippold W
Human complement factor B: functional properties of a recombinant zymogen of the alternative activation pathway convertase.
Immunobiology. 1993 Jul;188(3):221-32.
- PubMed ID
- 8225386 [ View in PubMed]
- Abstract
The human complement factor B is a centrally important component of the alternative pathway activation of the complement system. Here we report the isolation, characterization and eukaryotic expression of the first full length cDNA transcript for human factor B. In a factor B dependent haemolysis assay, the recombinant human factor B generated by transient COS cell transfection was shown to reconstitute haemolytic activity of factor B depleted human serum. To study the biological activities assigned to factor B, the availability of recombinant polypeptides representing definite portions of the human factor B molecule is desirable.