[Methods of hygromycin B phosphotransferase activity assay in transgenic plant].

Article Details

Citation

Zhuo Q, Yang X

[Methods of hygromycin B phosphotransferase activity assay in transgenic plant].

Wei Sheng Yan Jiu. 2004 Jul;33(4):502-4.

PubMed ID
15461291 [ View in PubMed
]
Abstract

Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the development of various transgenic plants, silence of inserted gene, marker-free system development and safety assessment of transgenic food. In this paper, several methods for detecting the activity of this enzyme were reviewed.

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