[Methods of hygromycin B phosphotransferase activity assay in transgenic plant].
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Zhuo Q, Yang X
[Methods of hygromycin B phosphotransferase activity assay in transgenic plant].
Wei Sheng Yan Jiu. 2004 Jul;33(4):502-4.
- PubMed ID
- 15461291 [ View in PubMed]
- Abstract
Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the development of various transgenic plants, silence of inserted gene, marker-free system development and safety assessment of transgenic food. In this paper, several methods for detecting the activity of this enzyme were reviewed.
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