Involvement of the system L amino acid transporter on uptake of S-nitroso-L-cysteine, an endogenous S-nitrosothiol, in PC12 cells.

Article Details

Citation

Nemoto T, Shimma N, Horie S, Saito T, Okuma Y, Nomura Y, Murayama T

Involvement of the system L amino acid transporter on uptake of S-nitroso-L-cysteine, an endogenous S-nitrosothiol, in PC12 cells.

Eur J Pharmacol. 2003 Jan 1;458(1-2):17-24.

PubMed ID
12498902 [ View in PubMed
]
Abstract

Previously, we proposed that S-nitroso-L-cysteine, an endogenous S-nitrosothiol, was incorporated via the system L-like amino acid transporter(s) in rat brain slices. In this study, we investigated the effect of S-nitroso-L-cysteine on L-[3H]leucine uptake in PC12 cells (a neuronal cell line). L-[3H]Leucine uptake in PC12 cells was Na(+) independent and significantly inhibited by an inhibitor of system L and by L-phenylalanine, L-cysteine, L-methionine and L-leucine at 1 mM. The effects of L-alanine, L-serine and L-threonine were limited. S-Nitroso-L-cysteine, but not other nitric oxide compounds, inhibited L-[3H]leucine uptake, and this inhibitory effect was eliminated by washing with buffer. System L is composed of the 4F2 light chains (LAT1 or LAT2) and the heavy chain, and the transcripts of these components were detected in RNA from PC12 cells. These findings suggest that S-nitroso-L-cysteine is incorporated via the system L amino acid transporter and thus regulates cell responses in PC12 cells.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
PhenylalanineLarge neutral amino acids transporter small subunit 2ProteinHumans
Unknown
Not AvailableDetails