Molecular cloning and characterization of prolyl endopeptidase from human T cells.

Article Details

Citation

Shirasawa Y, Osawa T, Hirashima A

Molecular cloning and characterization of prolyl endopeptidase from human T cells.

J Biochem. 1994 Apr;115(4):724-9.

PubMed ID
8089089 [ View in PubMed
]
Abstract

The prolyl endopeptidase (PEP) gene of human T cells was amplified by the PCR method and cloned in Escherichia coli. The complete gene consisted of 2,130 nucleotides corresponding to 710 amino acid residues with a calculated molecular mass of 80,750. The nucleotide sequence of this clone revealed that T cell PEP DNA is 48, 50, and 91% homologous to those of Flavobacterium meningosepticum, Aeromonas hydrophila, and porcine brain PEP, respectively. This gene was fused to the lacZ sequence from E. coli and expressed as a fused protein in E. coli. This fused protein exhibited PEP activity, which was inhibited by Z-Pro-prolinal, a specific inhibitor of PEP. The fused protein was purified on a beta-galactosidase specific affinity column. A polyclonal antibody was raised against the purified protein. Immunological characterization suggested that this protein is different from cytosol-soluble PEP.

DrugBank Data that Cites this Article

Polypeptides
NameUniProt ID
Prolyl endopeptidaseP48147Details