Esterase EstA
Details
- Name
- Esterase EstA
- Synonyms
- 3.1.1.1
- Autotransporter esterase EstA
- papA
- Gene Name
- estA
- Organism
- Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
- Amino acid sequence
>lcl|BSEQ0007617|Esterase EstA MIRMALKPLVAACLLASLSTAPQAAPSPYSTLVVFGDSLSDAGQFPDPAGPAGSTSRFTN RVGPTYQNGSGEIFGPTAPMLLGNQLGIAPGDLAASTSPVNAQQGIADGNNWAVGGYRTD QIYDSITAANGSLIERDNTLLRSRDGYLVDRARQGLGADPNALYYITGGGNDFLQGRILN DVQAQQAAGRLVDSVQALQQAGARYIVVWLLPDLGLTPATFGGPLQPFASQLSGTFNAEL TAQLSQAGANVIPLNIPLLLKEGMANPASFGLAADQNLIGTCFSGNGCTMNPTYGINGST PDPSKLLFNDSVHPTITGQRLIADYTYSLLSAPWELTLLPEMAHGTLRAYQDELRSQWQA DWENWQNVGQWRGFVGGGGQRLDFDSQDSAASGDGNGYNLTLGGSYRIDEAWRAGVAAGF YRQKLEAGAKDSDYRMNSYMASAFVQYQENRWWADAALTGGYLDYDDLKRKFALGGGERS EKGDTNGHLWAFSARLGYDIAQQADSPWHLSPFVSADYARVEVDGYSEKGASATALDYDD QKRSSKRLGAGLQGKYAFGSDTQLFAEYAHEREYEDDTQDLTMSLNSLPGNRFTLEGYTP QDHLNRVSLGFSQKLAPELSLRGGYNWRKGEDDTQQSVSLALSLDF
- Number of residues
- 646
- Molecular Weight
- 69608.325
- Theoretical pI
- 4.46
- GO Classification
- Functionscarboxylic ester hydrolase activity / lipase activityProcessesbacterial-type flagellum-dependent cell motility / bacterial-type flagellum-dependent swarming motility / biofilm formation / cell motility / glycolipid biosynthetic process / lipid biosynthetic process / single-species biofilm formationComponentscell outer membrane / integral component of membrane
- General Function
- Lipase activity
- Specific Function
- Esterase whose enzymatic activity is required for rhamnolipid production, all kinds of cell motility (swimming, swarming, and twitching), and biofilm formation; the exact role of EstA in these processes is unclear. In vitro, has pronounced esterase activities towards p-nitrophenyl esters of short acyl chain length (C4-C6) and Tween detergents. Also shows relatively high activity towards beta-naphthyl butyrate, whereas its activities towards triacylglycerols and acyls-CoA are negligible.
- Pfam Domain Function
- Transmembrane Regions
- 398-408 411-421 438-447 452-461 489-500 508-518 548-558 562-571 606-615 619-628 637-646
- Cellular Location
- Cell outer membrane
- Gene sequence
>lcl|BSEQ0012772|Esterase EstA (estA) ATGATCAGAATGGCGCTCAAGCCACTGGTAGCGGCCTGCCTGCTGGCTTCGCTGTCCACC GCCCCGCAGGCTGCTCCTTCGCCCTATTCGACGCTGGTGGTGTTCGGCGACAGCCTCAGC GATGCCGGGCAGTTCCCCGATCCTGCCGGCCCCGCCGGAAGCACCTCGCGTTTCACCAAC CGGGTCGGCCCGACCTACCAGAACGGCAGCGGCGAGATCTTCGGACCGACCGCGCCCATG CTGCTCGGCAATCAGCTCGGCATCGCCCCGGGTGACCTGGCTGCCTCGACCTCGCCGGTC AACGCCCAGCAGGGCATCGCCGACGGCAACAACTGGGCGGTGGGCGGCTACCGGACCGAT CAGATCTACGACTCGATCACCGCGGCCAACGGCTCGCTGATCGAGCGCGACAATACCCTC CTGCGCAGCCGCGATGGCTACCTGGTGGACCGTGCCCGCCAGGGCCTGGGTGCCGACCCG AACGCGTTGTACTACATCACCGGCGGCGGCAACGACTTTCTCCAGGGGCGCATCCTCAAC GACGTCCAGGCCCAACAGGCCGCCGGTCGCCTGGTGGATAGCGTGCAGGCCCTGCAGCAG GCCGGCGCGCGCTACATCGTGGTCTGGCTGTTGCCCGACCTGGGCCTGACCCCGGCTACC TTCGGTGGTCCCTTGCAGCCTTTCGCCAGCCAACTCAGCGGCACGTTCAACGCCGAGCTG ACCGCCCAGTTGAGCCAGGCCGGCGCCAACGTCATTCCGTTGAACATCCCGCTGCTGCTC AAGGAAGGCATGGCCAACCCGGCTTCCTTCGGCCTGGCCGCCGACCAGAACCTGATCGGC ACCTGTTTCAGCGGCAACGGCTGCACCATGAACCCGACCTACGGGATCAACGGCAGCACG CCCGACCCGAGCAAATTGCTGTTCAACGACAGCGTGCACCCGACCATCACCGGCCAGCGC CTGATCGCCGACTACACCTATTCGCTGCTGTCGGCGCCCTGGGAGCTGACCCTGCTGCCG GAAATGGCCCACGGCACCCTGCGTGCCTACCAGGACGAACTGCGCAGCCAGTGGCAGGCG GACTGGGAGAACTGGCAGAACGTCGGCCAGTGGCGCGGCTTCGTCGGCGGCGGTGGCCAG CGCCTGGACTTCGACTCCCAGGACAGCGCCGCCAGCGGCGACGGCAACGGCTACAACCTG ACCCTTGGTGGCAGCTACCGCATCGACGAGGCCTGGCGCGCCGGGGTCGCCGCCGGTTTC TACCGGCAGAAGCTGGAAGCCGGCGCCAAGGATTCCGACTACCGGATGAACAGCTACATG GCCAGCGCCTTCGTGCAGTACCAGGAAAACCGCTGGTGGGCCGACGCGGCGTTGACCGGC GGCTACCTCGACTACGACGACCTGAAGCGCAAGTTCGCCCTGGGCGGCGGCGAGCGCAGC GAGAAAGGCGACACCAACGGCCACCTGTGGGCGTTCAGCGCGCGCCTGGGCTACGACATC GCCCAGCAGGCCGACAGTCCCTGGCACCTGTCGCCGTTCGTCAGCGCCGACTATGCACGG GTCGAGGTCGACGGCTATTCCGAGAAGGGCGCCAGCGCCACCGCGCTCGACTACGACGAC CAGAAGCGCAGCTCGAAGCGCCTGGGCGCCGGCCTGCAAGGCAAGTACGCGTTCGGCAGC GATACCCAGCTGTTCGCCGAGTACGCCCACGAACGTGAGTACGAGGACGACACCCAGGAC CTGACCATGTCCCTCAACAGCCTGCCGGGCAATCGCTTCACCCTCGAAGGCTACACCCCG CAGGACCATCTCAACCGCGTCTCACTCGGCTTCAGCCAGAAGCTGGCGCCGGAGCTGTCG CTGCGCGGCGGCTACAACTGGCGCAAGGGCGAGGACGATACCCAGCAGAGCGTCAGCCTG GCGCTGAGCCTGGACTTCTGA
- Chromosome Location
- Not Available
- Locus
- Not Available
- External Identifiers
Resource Link UniProtKB ID O33407 UniProtKB Entry Name ESTA_PSEAE GenBank Protein ID 2218156 GenBank Gene ID AF005091 - General References
- Wilhelm S, Tommassen J, Jaeger KE: A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa. J Bacteriol. 1999 Nov;181(22):6977-86. [Article]
- Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [Article]
- Wilhelm S, Gdynia A, Tielen P, Rosenau F, Jaeger KE: The autotransporter esterase EstA of Pseudomonas aeruginosa is required for rhamnolipid production, cell motility, and biofilm formation. J Bacteriol. 2007 Sep;189(18):6695-703. Epub 2007 Jul 13. [Article]
- Lescic Asler I, Ivic N, Kovacic F, Schell S, Knorr J, Krauss U, Wilhelm S, Kojic-Prodic B, Jaeger KE: Probing enzyme promiscuity of SGNH hydrolases. Chembiochem. 2010 Oct 18;11(15):2158-67. doi: 10.1002/cbic.201000398. [Article]
- van den Berg B: Crystal structure of a full-length autotransporter. J Mol Biol. 2010 Feb 26;396(3):627-33. doi: 10.1016/j.jmb.2009.12.061. Epub 2010 Jan 11. [Article]