Dihydrofolate reductase

Details

Name
Dihydrofolate reductase
Synonyms
  • 1.5.1.3
Gene Name
DHFR
Organism
Gallus gallus
Amino acid sequence
>lcl|BSEQ0052482|Dihydrofolate reductase
VRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSI
PEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAV
YKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEEDGIQYKFE
VYQKSVLAQ
Number of residues
189
Molecular Weight
21649.77
Theoretical pI
Not Available
GO Classification
Functions
dihydrofolate reductase activity / drug binding / mRNA binding / NADP binding
Processes
dihydrofolate metabolic process / folic acid metabolic process / one-carbon metabolic process / response to methotrexate / tetrahydrofolate biosynthetic process / tetrahydrofolate metabolic process
Components
mitochondrion
General Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. May bind to mRNA.
Specific Function
Dihydrofolate reductase activity
Pfam Domain Function
Transmembrane Regions
Not Available
Cellular Location
Not Available
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP00378
UniProtKB Entry NameDYR_CHICK
General References
  1. Kumar AA, Blankenship DT, Kaufman BT, Freisheim JH: Primary structure of chicken liver dihydrofolate reductase. Biochemistry. 1980 Feb 19;19(4):667-78. doi: 10.1021/bi00545a010. [Article]
  2. Fan YX, Ju M, Zhou JM, Tsou CL: Activation of chicken liver dihydrofolate reductase by urea and guanidine hydrochloride is accompanied by conformational change at the active site. Biochem J. 1996 Apr 1;315 ( Pt 1):97-102. doi: 10.1042/bj3150097. [Article]
  3. McTigue MA, Davies JF 2nd, Kaufman BT, Kraut J: Crystal structure of chicken liver dihydrofolate reductase complexed with NADP+ and biopterin. Biochemistry. 1992 Aug 18;31(32):7264-73. doi: 10.1021/bi00147a009. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03904Ureaapproved, investigationalyesactivatorDetails