Dihydrofolate reductase

Details

Name
Dihydrofolate reductase
Synonyms
  • 1.5.1.3
Gene Name
DHFR
UniProtKB Entry
P00378Swiss-Prot
Organism
Gallus gallus
NCBI Taxonomy ID
9031
Amino acid sequence
>lcl|BSEQ0052482|Dihydrofolate reductase
VRSLNSIVAVCQNMGIGKDGNLPWPPLRNEYKYFQRMTSTSHVEGKQNAVIMGKKTWFSI
PEKNRPLKDRINIVLSRELKEAPKGAHYLSKSLDDALALLDSPELKSKVDMVWIVGGTAV
YKAAMEKPINHRLFVTRILHEFESDTFFPEIDYKDFKLLTEYPGVPADIQEEDGIQYKFE
VYQKSVLAQ
Number of residues
189
Molecular Weight
21649.77
Theoretical pI
Not Available
GO Classification
Functions
dihydrofolate reductase activity / drug binding / mRNA binding / NADP binding
Processes
dihydrofolate metabolic process / folic acid metabolic process / one-carbon metabolic process / response to methotrexate / tetrahydrofolate biosynthetic process / tetrahydrofolate metabolic process
Components
mitochondrion
General Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. May bind to mRNA.
Specific Function
Dihydrofolate reductase activity
Pfam Domain Function
Signal Regions
Not Available
Transmembrane Regions
Not Available
Cellular Location
Not Available
Gene sequence
Not Available
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP00378
UniProtKB Entry NameDYR_CHICK
PDB ID(s)1DR1, 1DR2, 1DR3, 1DR4, 1DR5, 1DR6, 1DR7, 8DFR
General References
  1. Kumar AA, Blankenship DT, Kaufman BT, Freisheim JH: Primary structure of chicken liver dihydrofolate reductase. Biochemistry. 1980 Feb 19;19(4):667-78. doi: 10.1021/bi00545a010. [Article]
  2. Fan YX, Ju M, Zhou JM, Tsou CL: Activation of chicken liver dihydrofolate reductase by urea and guanidine hydrochloride is accompanied by conformational change at the active site. Biochem J. 1996 Apr 1;315 ( Pt 1):97-102. doi: 10.1042/bj3150097. [Article]
  3. McTigue MA, Davies JF 2nd, Kaufman BT, Kraut J: Crystal structure of chicken liver dihydrofolate reductase complexed with NADP+ and biopterin. Biochemistry. 1992 Aug 18;31(32):7264-73. doi: 10.1021/bi00147a009. [Article]

Associated Data

Bio-Entities
Bio-EntityType
Dihydrofolate reductase (Gallus gallus)protein
primary
Drug Relations
DrugDrug groupPharmacological action?TypeActionsDetails
Ureaapproved, investigationalyestargetactivatorDetails