Outer membrane protein TolC

Details

Name
Outer membrane protein TolC
Synonyms
  • colE1-i
  • mtcB
  • mukA
  • Multidrug efflux pump subunit TolC
  • Outer membrane factor TolC
  • refI
  • toc
  • weeA
Gene Name
tolC
Organism
Escherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0003825|Outer membrane protein TolC
MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAADRDAAFEKINEARSPL
LPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSKWRALTLQEKAAGIQDVTY
QTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLDQTTQRFNVGLVAITDVQNAR
AQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAALNVENFKTDKPQPVNALLKEAEK
RNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTASTGISDTSYSGSKTRGAAGTQYDDSN
MGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGASEQLESAHRSVVQTVRSSFNNINASI
SSINAYKQAVVSAQSSLDAMEAGYSVGTRTIVDVLDATTTLYNAKQELANARYNYLINQL
NIKSALGTLNEQDLLALNNALSKPVSTNPENVAPQTPEQNAIADGYAPDSPAPVVQQTSA
RTTTSNGHNPFRN
Number of residues
493
Molecular Weight
53740.24
Theoretical pI
5.3
GO Classification
Functions
efflux transmembrane transporter activity / identical protein binding / porin activity
Processes
enterobactin transport / response to antibiotic / response to organic cyclic compound / transmembrane transport
Components
cell outer membrane / integral component of membrane
General Function
Porin activity
Specific Function
Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.
Pfam Domain Function
Transmembrane Regions
63-74 83-96 269-279 301-311
Cellular Location
Cell outer membrane
Gene sequence
>lcl|BSEQ0019205|Outer membrane protein TolC (tolC)
ATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGTTCGTTGAGC
CAGGCCGAGAACCTGATGCAAGTTTATCAGCAAGCACGCCTTAGTAACCCGGAATTGCGT
AAGTCTGCCGCCGATCGTGATGCTGCCTTTGAAAAAATTAATGAAGCGCGCAGTCCATTA
CTGCCACAGCTAGGTTTAGGTGCAGATTACACCTATAGCAACGGCTACCGCGACGCGAAC
GGCATCAACTCTAACGCGACCAGTGCGTCCTTGCAGTTAACTCAATCCATTTTTGATATG
TCGAAATGGCGTGCGTTAACGCTGCAGGAAAAAGCAGCAGGGATTCAGGACGTCACGTAT
CAGACCGATCAGCAAACCTTGATCCTCAACACCGCGACCGCTTATTTCAACGTGTTGAAT
GCTATTGACGTTCTTTCCTATACACAGGCACAAAAAGAAGCGATCTACCGTCAATTAGAT
CAAACCACCCAACGTTTTAACGTGGGCCTGGTAGCGATCACCGACGTGCAGAACGCCCGC
GCACAGTACGATACCGTGCTGGCGAACGAAGTGACCGCACGTAATAACCTTGATAACGCG
GTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTC
GAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAA
CGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGC
CAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGAC
ACCTCTTATAGCGGTTCGAAAACCCGTGGTGCCGCTGGTACCCAGTATGACGATAGCAAT
ATGGGCCAGAACAAAGTTGGCCTGAGCTTCTCGCTGCCGATTTATCAGGGCGGAATGGTT
AACTCGCAGGTGAAACAGGCACAGTACAACTTTGTCGGTGCCAGCGAGCAACTGGAAAGT
GCCCATCGTAGCGTCGTGCAGACCGTGCGTTCCTCCTTCAACAACATTAATGCATCTATC
AGTAGCATTAACGCCTACAAACAAGCCGTAGTTTCCGCTCAAAGCTCATTAGACGCGATG
GAAGCGGGCTACTCGGTCGGTACGCGTACCATTGTTGATGTGTTGGATGCGACCACCACG
TTGTACAACGCCAAGCAAGAGCTGGCGAATGCGCGTTATAACTACCTGATTAATCAGCTG
AATATTAAGTCAGCTCTGGGTACGTTGAACGAGCAGGATCTGCTGGCACTGAACAATGCG
CTGAGCAAACCGGTTTCCACTAATCCGGAAAACGTTGCACCGCAAACGCCGGAACAGAAT
GCTATTGCTGATGGTTATGCGCCTGATAGCCCGGCACCAGTCGTTCAGCAAACATCCGCA
CGCACTACCACCAGTAACGGTCATAACCCTTTCCGTAACTGA
Chromosome Location
Not Available
Locus
Not Available
External Identifiers
ResourceLink
UniProtKB IDP02930
UniProtKB Entry NameTOLC_ECOLI
GenBank Protein ID43107
GenBank Gene IDX54049
General References
  1. Hackett J, Reeves P: Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12. Nucleic Acids Res. 1983 Sep 24;11(18):6487-95. [Article]
  2. Niki H, Imamura R, Ogura T, Hiraga S: Nucleotide sequence of the tolC gene of Escherichia coli. Nucleic Acids Res. 1990 Sep 25;18(18):5547. [Article]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [Article]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [Article]
  5. Hackett J, Misra R, Reeves P: The TolC protein of Escherichia coli K12 is synthesised in a precursor form. FEBS Lett. 1983 Jun 13;156(2):307-10. [Article]
  6. Morona R, Manning PA, Reeves P: Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. J Bacteriol. 1983 Feb;153(2):693-9. [Article]
  7. Link AJ, Robison K, Church GM: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis. 1997 Aug;18(8):1259-313. [Article]
  8. Molloy MP, Herbert BR, Walsh BJ, Tyler MI, Traini M, Sanchez JC, Hochstrasser DF, Williams KL, Gooley AA: Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 1998 May;19(5):837-44. [Article]
  9. Koronakis V, Li J, Koronakis E, Stauffer K: Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals. Mol Microbiol. 1997 Feb;23(3):617-26. [Article]
  10. Kobayashi K, Tsukagoshi N, Aono R: Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element. J Bacteriol. 2001 Apr;183(8):2646-53. [Article]
  11. Touze T, Eswaran J, Bokma E, Koronakis E, Hughes C, Koronakis V: Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system. Mol Microbiol. 2004 Jul;53(2):697-706. [Article]
  12. Stenberg F, Chovanec P, Maslen SL, Robinson CV, Ilag LL, von Heijne G, Daley DO: Protein complexes of the Escherichia coli cell envelope. J Biol Chem. 2005 Oct 14;280(41):34409-19. Epub 2005 Aug 3. [Article]
  13. Lin HT, Bavro VN, Barrera NP, Frankish HM, Velamakanni S, van Veen HW, Robinson CV, Borges-Walmsley MI, Walmsley AR: MacB ABC transporter is a dimer whose ATPase activity and macrolide-binding capacity are regulated by the membrane fusion protein MacA. J Biol Chem. 2009 Jan 9;284(2):1145-54. doi: 10.1074/jbc.M806964200. Epub 2008 Oct 27. [Article]
  14. Symmons MF, Bokma E, Koronakis E, Hughes C, Koronakis V: The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7173-8. doi: 10.1073/pnas.0900693106. Epub 2009 Apr 2. [Article]
  15. Tikhonova EB, Dastidar V, Rybenkov VV, Zgurskaya HI: Kinetic control of TolC recruitment by multidrug efflux complexes. Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16416-21. doi: 10.1073/pnas.0906601106. Epub 2009 Sep 2. [Article]
  16. Xu Y, Song S, Moeller A, Kim N, Piao S, Sim SH, Kang M, Yu W, Cho HS, Chang I, Lee K, Ha NC: Functional implications of an intermeshing cogwheel-like interaction between TolC and MacA in the action of macrolide-specific efflux pump MacAB-TolC. J Biol Chem. 2011 Apr 15;286(15):13541-9. doi: 10.1074/jbc.M110.202598. Epub 2011 Feb 16. [Article]
  17. Fontaine F, Fuchs RT, Storz G: Membrane localization of small proteins in Escherichia coli. J Biol Chem. 2011 Sep 16;286(37):32464-74. doi: 10.1074/jbc.M111.245696. Epub 2011 Jul 21. [Article]
  18. Zakharov SD, Sharma O, Zhalnina M, Yamashita E, Cramer WA: Pathways of colicin import: utilization of BtuB, OmpF porin and the TolC drug-export protein. Biochem Soc Trans. 2012 Dec 1;40(6):1463-8. doi: 10.1042/BST20120211. [Article]
  19. Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C: Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature. 2000 Jun 22;405(6789):914-9. [Article]
  20. Higgins MK, Eswaran J, Edwards P, Schertler GF, Hughes C, Koronakis V: Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug efflux protein TolC. J Mol Biol. 2004 Sep 17;342(3):697-702. [Article]
  21. Bavro VN, Pietras Z, Furnham N, Perez-Cano L, Fernandez-Recio J, Pei XY, Misra R, Luisi B: Assembly and channel opening in a bacterial drug efflux machine. Mol Cell. 2008 Apr 11;30(1):114-21. doi: 10.1016/j.molcel.2008.02.015. [Article]
  22. Pei XY, Hinchliffe P, Symmons MF, Koronakis E, Benz R, Hughes C, Koronakis V: Structures of sequential open states in a symmetrical opening transition of the TolC exit duct. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2112-7. doi: 10.1073/pnas.1012588108. Epub 2011 Jan 18. [Article]

Drug Relations

Drug Relations
DrugBank IDNameDrug groupPharmacological action?ActionsDetails
DB03350Cobalt hexammine ionexperimentalunknownDetails